| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Gretna Elite Academy GEA Elite 05/06B |
02077-0011B9U-0059 | Zach Barnes |
| A2 | Sting Soccer Sting NE 05B Bergt ECNL-RL |
03137-0011B9U-0057 | Joel Bergt |
| A3 | Sporting Nebraska FC Sporting Nebr B ECNL 05/06 |
03036-0011B9U-0055 | Mark Heath-Preston |
| A4 | Sporting Nebraska FC Sporting Nebr B ECNL-RL 05/06 |
03036-0011B9U-0061 | Paul Grandgenett |
| A5 | Villarreal Nebraska Academy VNA HS Boys Amarillo |
03131-0011B9U-0001 | Jorge Sambataro |
| A6 | Dreamers FC DFC Aztecs 06 |
03134-0011B8U-1793 | Johnny Gress |
| A7 | Sting Soccer Sting NE 07B Beckmann ECNL-RL |
03137-0011B7U-0062 | James Beckmann |
| A8 | Sporting Nebraska FC Sporting Nebr B ECNL 2007 |
03036-0011B7U-0051 | Caleb Hinson |
| A9 | Omaha United Soccer Club Omaha United SC 2006B Black |
03133-0011B8U-0002 | Randall Esquivel |
| A10 | Evolution Soccer Club Evolution 05/06 Orange - B19U |
03099-0011B9U-0160 | Christopher Brunt |
| A11 | Omaha Housing Authority OHA-SFA Jets 05-06 |
03082-0011B9U-1113 | Osuman Issaka |
| A12 | Interstate Soccer Club ISC 2006B Blue |
04058-0011B8U-0002 | Osbin Rogel |
| A13 | Evolution Soccer Club EVO WOL Bears - B18U |
03099-0011B8U-0161 | Daniel Vasquez Gutierrez |