| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Gretna Elite Academy GEA Academy Pre-ECNL II 15B |
02077-0012B9U-0122 | Logan Mendez |
| A2 | Eagles Football Club Eagles Football Club - B9U Gold |
05087-0012B9U-0009 | Gustabo Vazquez |
| A3 | Villarreal Nebraska Academy VNA 2015 Boys Amarillo |
03131-0013B9U-0016 | Jose Hernandez |
| A4 | Sporting Nebraska FC Sporting Nebr B Academy Leeds 2015 |
03036-0012B9U-2297 | Caleb Brown |
| A5 | Sting Soccer Sting NE 15B Tungland |
03137-0012B9U-0097 | Demetri Fountas |
| A6 | Omaha United Soccer Club Omaha United SC 2015B White |
03133-0012B9U-0055 | Jovan Sudar |
| A7 | Sporting Nebraska FC Sporting Nebr B Pre Acad Celtic 2016 |
03036-0012B8U-2329 | Danny Gornell |
| B1 | Bennington Soccer Club BENSC Lightning 9B |
03057-0013B9U-0028 | Tom Miller |
| B2 | Sporting Nebraska FC Sporting Nebr B Pre Acad Rangers 2016 |
03036-0012B8U-2328 | Danny Gornell |
| B3 | Columbus Soccer Club CSC Destroyers 2015B |
04017-0012B9U-2036 | Joe Citta |
| B4 | Heartland FC HFC Freeze 9U |
05085-0012B9U-0001 | Josh Berlie |
| B6 | United Association Football UAF 2015B Blue |
03001-0012B9U-0021 | Mary Matthews |
| B7 | Gretna Elite Academy GEA Academy Pre-ECNL I 16B |
02077-0012B9U-0127 | Raphael Martinez |