| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Villarreal Nebraska Academy VNA 2015 Boys Roda |
03131-0013B9U-0024 | Otan Arrey Arrey |
| A2 | Gretna Elite Academy GEA Academy White II 15B |
02077-0013B9U-0096 | Ariel Ayala-Reyes |
| A3 | Sporting Nebraska FC Sporting Nebr B Waverly Black 2015 |
03036-001YFB9U-2325 | Derek Bash |
| A4 | United Association Football UAF 2015B Gray |
03001-0012B9U-0020 | Amber Allen |
| A5 | Beatrice Nighthawks Beatrice Nighthawks - B5 |
03060-0013B9U-0100 | Manalle Armstrong |
| A6 | Blair Soccer Club Blair FC Nitro |
04019-0013B9U-1083 | Michaela McCarty |
| A7 | St Columbkille Soccer Club Crusaders 9UB |
02071-0013B9U-0047 | Ryan Howery |